Chitinase gene of Capsicum annuum L. cv. Hanbyul and probing method of resistance for plant disease

摘要

PURPOSE: Provided are a chitinase gene of Capsicum annuum L. cv. Hanbyul and a probing method of resistance to plant diseases, such as Xanthomonas campestrispv. vesicatoria. CONSTITUTION: The gene of chitinase having a resistance mechanism to Xanthomonas campestrispv. vesicatoria is isolated from Capsicum annuumL. cv. Hanbyul by the steps of: inoculating Xanthomonas campestrispv. vesicatoria into Capsicum annuumL. cv. Hanbyul; isolating mRNA from the leaves of infected Capsicum annuumL. cv. Hanbyul then preparing cDNA by reverse transcription, and primers; performing PCR with them to prepare cDNA library and screening chitinase gene therefrom; then sequencing its amino acid sequence, wherein the amino acid sequence is represented by figure(1).