20 A purification method of pravastatin precursor compactin and derivatives thereof using HP20 resin and methanol

摘要

PURPOSE: Provided is a purification method of pravastatin precursor compactin and derivatives thereof particularly by absorption chromatography using HP20 resin. CONSTITUTION: The purification method of pravastatin precursor compactin and its derivatives comprises the steps of: fermenting strains producing compactin and its derivatives to obtain a fermentation solution; performing absorption chromatography with the fermentation solution using methanol and HP20 resin; and recovering pravastatin precursor compactin and its derivatives by using methanol, wherein the absorption chromatography is performed at 0-20% of the methanol content in absorption solution with maintaining the methanol content in eluting solution to 60% or more.