Method and apparatus for the detection of fluorescence light in the case of the confocal scanning microscopy

摘要

The present invention relates to a method and a device for the detection of fluorescence light in the case of the scanning microscopy, in particular in the confocal scanning microscopy, wherein fluorescent materials be excited to fluoresce by multiphoton excitation, and is used for the optimization of the object detection, characterized in that, for the optimum fluorescent photon yield which the operating parameters of the multi-photon excitation light source (2) and / or the system parameters of the confocal raster microscope on the properties of the fluorescent materials are adapted to.