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New polynucleotide encoding the CysQ transporter of coryneform bacteria, useful, when over expressed, for increasing fermentative production of amino acids
New polynucleotide encoding the CysQ transporter of coryneform bacteria, useful, when over expressed, for increasing fermentative production of amino acids
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机译:编码棒状细菌CysQ转运蛋白的新多核苷酸,在过量表达时可用于增加氨基酸的发酵产量
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摘要
Isolated polynucleotide (I) from coryneform bacteria (i) is at least 70% identical with a sequence that encodes a 252 amino acid (aa) polypeptide (2), reproduced. (I) may encode a polypeptide (ii) at least 70% identical with (2). (I) can be the complement (iii) of (i) or (ii); or is a chain (iv) that contains at least 15 consecutive nucleotides from (i)-(iii). Isolated polynucleotide (I) from coryneform bacteria (i) is at least 70% identical with a sequence that encodes a 252 amino acid (aa) polypeptide (2), reproduced. (I) may encode a polypeptide (ii) at least 70% identical with (2). (I) can be the complement (iii) of (i) or (ii); or is a chain (iv) that contains at least 15 consecutive nucleotides from (i)-(iii). The polypeptides preferably have the activity of the CysQ transport protein. Independent claims are also included for the following: (a) coryneform bacteria in which activity of the cysQ gene has been increased, especially by overexpression; (b) method for fermentative production of L-aa, especially L-Lys, L-Cys and L-Met, by culturing cells of (a); and (c) coryneform bacteria carrying a vector that contains (I).
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