Polymerase chain reaction mixture, useful e.g. for genomic analysis, includes bovine serum albumin, allowing reduction in concentration of polymerase and fluorescent reagents

摘要

Polymerase chain reaction (PCR) mixture (A) for fluorescent-based gene expression and gene mutation analysis comprising: a Taq polymerase (I); bovine serum albumin (BSA) at final concentration 400-800 mu g/ml; a high content of magnesium chloride, depending on the (I) used, the final concentration is over 3.5 to 5 mM; and a fluorescent material (II).