embryonic stem cells of ungulates as kerndonatoren and kerntransfertechniken chimeric and transgenic animals for the production

摘要

The use of embryonic stem (ES) cells as donor nuclei for nuclear transfer (NT) procedures is provided. The method provides for the production of ES cell derived NT embryos. The use of ES cell derived NT embryos for producing chimeric non-human animals, preferably ungulates is also provided. Additionally, the use of transgenic ES cells as nuclear donors to provide transgenic embryos and transgenic offspring is taught.