A process for the production of human or human die lysine by means of recombinant dna.

摘要

PURPOSE: To obtain the subject compound useful as a reagent, etc., for research capable of separating a cell from an animal tissue on an industrial scale by efficiently expressing a human matrilysin gene in microorganism, separating and purifying the product from a bacterial cell of the microorganism. ;CONSTITUTION: A gene coding a sequence of a signal peptide of an alkaline phosphatase of E. coli is bound to the N-terminal side of an amino acid sequence of matrilysin and a gene of human matrilysin containing a base sequence expressed by the formula is integrated into a plasmid vector to prepare a recombinant plasmid pλPR-MAT and the plasmid is transduced into a host such as a microorganism, E. coli, etc., to transform the host and the transformed host is cultured to efficiently express a gene and a product is separated and purified from the transformed microbial cell of the microorganism or the culture medium to simply provide matrilysin useful as reagent for research of medical chemistry, biochemistry and pharmacy to be used for peeling various animal culturing cells from vessel wall or separating a cell from various animal tissues.;COPYRIGHT: (C)1996,JPO