detection and identification of human papillomavirus by pcr and type specific reverse hybridization

摘要

The present invention relates to a method for detection and/or identification of HPV present in a biological sample, comprising the steps of amplification of HPV polynucleic acids and of hybridization of said amplified polynucleic acids to a number of probes. By means of PCR, a short fragment of the L1 gene of HPV is amplified. The amplimers are then contacted with probes that specifically hybridize to said short fragment of the L1 gene of either one or more than one HPV type. A preferred format is the reverse hybridization technique, more particularly the LiPA technique. The invention also relates to primers and probes to be used in a method of detection and/or identification of HPV and to a diagnostic kit to perform said method. The invention further relates to new HPV sequences.