METHOD OF DETECTING ACTIVATORS OR INHIBITORS OF RECEPTORS OF THE INSULIN RECEPTOR FAMILY USING AN ISOLATED CHIMERIC RECEPTOR

摘要

The invention concerns a method for detecting activator and inhibitor compounds of receptors of the family of the insulin receptor by means of an isolated chimeric receptor, comprising a first alpha beta heterodimer, associated with a first response element and a second alpha beta heterodimer associated with a second response element different from the first, said first response element being capable of acting on the second response element when the two are brought close together. The invention also concerns such an isolated chimeric receptor, compositions containing it, and fusion cDNA's, expression vectors comprising said cDNA's and cells genetically modified by transfection of said expression vectors for expressing said chimeric receptor in a cell. The invention further concerns the use of the activator and inhibitor compounds of the family of the insulin receptor capable of binding with said isolated chimeric receptor for preparing a medicine designed for the treatment of human and/or animal pathologies or a medicine designed for tissue regeneration or still for preparing growth factors for cell culture.