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Chemical signal reinforcement protein - inside the cell of dynamic strength - be su for the medicine screening and concerning the fluorescent group - based re-distribution assay
Chemical signal reinforcement protein - inside the cell of dynamic strength - be su for the medicine screening and concerning the fluorescent group - based re-distribution assay
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机译:化学信号增强蛋白-动态强度的细胞内-可用于药物筛选并涉及基于荧光基团的重新分布测定
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摘要
The present invention involves that adding certain dyes to the solution outside cells expressing a GFP-tagged proteins in a redistribution assay, enhance the signal. Examples of such dyes are Trypan Blue and Acid Red. This enables redistribution assays to be measured as a change in light intensity. Redistribution movement from the cytosol to the membrane, or vice versa, are illustrated. The apparatus used for measuring the redistribution is an ordinary plate reader or a FLIPRTM type instrument. It is a common feature among the dyes described that the enhancer compound has an absorption spectrum overlapping the emission spectrum of the GFP.
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