Novel isoform of human cAMP-dependent protein kinase, Cα-s, is localized in sperm half-pieces and uses thereof

摘要

The cloning of a novel human Calpha isoform, identified as the human Calpha-s protein on the basis of its similarity to a recently characterized Calpha isoform which was identified by purification and peptide sequencing of Calpha from ovine sperm. The human Calpha-s protein was present in testis and ejaculated sperm and Calpha-s localized to the midpiece of human sperm. A cDNA sequence encodes a Calpha-s protein and includes the specific nucleotide sequence shown in SEQ ID NO:1, wherein the protein is a new splice variant of Calpha catalytic subunit of cAMP dependent protein kinase.