DNA polymerase mutant having a mutation of one or more active site

摘要

(57) A variant of the natural DNA polymerases, [Abstract] The present invention provides one more amino acids are mutated or one of its active sites. DNA polymerase variant of the invention has an enzyme activity that has been modified and fidelity that have changed compared to native DNA polymerase. Eg, DNA polymerase variant exhibits a fidelity dDNP / rNTP specificity of the enzyme increased activity, has been changed, or increased. In one embodiment of the present invention, DNA polymerases of the natural amino acid sequence motif in the active site: comprising Asp Tyr Ser Gln Ile glu Leu Arg. In another aspect of the present invention, DNA Porimerase of the natural amino acid sequence motif in the active site: have a Leu Leu Val Ala Leu Asp Tyr Ser Gln Ile Glu Leu Arg. The amino acid substitutions of one or more of (a) the amino acid sequence motifs or mutant DNA polymerase is modified by substitution of amino acids other than Glu (b) the amino acid sequence motif in the active site of the native DNA polymerase.