Used for the measurement of control cAPM subunit cAPM dependent protein kinase from the Cu Chi di osteoprotegerin of helium (PKA)

摘要

(57) [summary] The present invention relates to the use for the detection of control cAPM subunit cAPM dependent protein kinase from the Cu Chi di osteoprotegerin Corynebacterium Jisukoideumu of (PKA). The present invention encompasses a construct for green fluorescent protein fusion to (GFP) and expression in E. coli of R- subunit. By using a mutant GFP, emission spectrum or absorption, or use or cGMP cAPM fluorescently labeled modified and used as a means for monitoring cAPM join (FRET) Fluorescence energy transfer. changes in FRET due cAPM bond, will allow the measurement in living cells or in vitro cAPM level.