How to obtain a full-length nucleic acid sequences using the Escherichia coli and homologues topoisomerase III

摘要

(57) [summary] The present invention encompasses a method for obtaining the full-length coding cDNA. The use for the synthesis of first strand cDNA full length mRNA isolated and binds to the cDNA unnatural tag, the method of the present invention is used for the synthesis of second strand cDNA first strand was tagged It encompasses. The amplified cDNA is further last, can be inserted into an expression vector. Also disclosed are nucleic acid constructs containing the full length cDNA coding and full-length coding cDNA isolated was prepared using the method of the present invention.