ELECTROPHORESIS METHOD OPTIMIZED TO GRAIN PROTEIN, GEL FOR ELECTROPHORESIS USED THEREFOR, BUFFER SOLUTION, AND SAMPLE EXTRACT

摘要

PROBLEM TO BE SOLVED: To achieve a clear migration pattern in a shorter time than by a conventional method when electrophoresis is performed on grain proteins.;SOLUTION: Eelectrophoresis is performed on the grain protein with three essential elements of (1) a polyacrylamide gel of which the maximum degree of swelling in water is between 1.3-2.0, (2) a sample extract for SDS-PAGE containing of a thiolic reducer which corresponds to a large excess amount to the number of cysteine residual groups in the sample proteins in a buffer solution of pH 7.0-9.0, and (3) a migration buffer solution both having negative charge while holding SH groups in a migration buffer solution (tris (hydroxymethyl) aminomethane of 25 mM, glycine of 192 mM, (w/v) SDS of 0.1%) of Laemmli method composition, a discontinuous buffer solution of SDS- PAGE, and containing the thiolic reducer to be developed into the gel during electrophoresis.;COPYRIGHT: (C)2004,JPO