首页> 外国专利> MICRO PROTEIN ELECTROPHORETIC APPARATUS DUE TO SILVER STAINING TECHNIQUES

MICRO PROTEIN ELECTROPHORETIC APPARATUS DUE TO SILVER STAINING TECHNIQUES

机译:由于银染色技术而产生的微蛋白质电泳仪

摘要

PROBLEM TO BE SOLVED: To solve the problem that considerable labor and time are required and analysis cannot be made by routine work since operation procedures are complicated in an existing micro protein electrophoretic analysis in a polymer porous film due to manipulation, and to provide an automatic micro protein electrophoretic apparatus that can automatically and simultaneously carry out a separation analysis due to micro protein electrophoretic in the polymer porous film to a number of specimens.;SOLUTION: When the apparatus is started up, a support is automatically wetted by a buffer liquid, a fixed amount of specimen is applied onto the support that is wetted by the buffer liquid, the spacemen that is applied onto the support migrates, a migration image on the support appearing due to migration is fixed, a protein-fixing liquid contained in the support is washed, the migration image on the washed support is subjected to silver staining techniques, the stained support is decolorized, silver is stuck to the migration image on the decolorized support, and the concentration of the migration image is measured.;COPYRIGHT: (C)2003,JPO
机译:要解决的问题:解决现有的高分子多孔膜微蛋白质电泳分析中由于操作而使操作步骤变得复杂的问题,从而需要大量的劳动和时间并且无法通过常规工作进行分析,并提供一种自动方法。微蛋白电泳仪,由于在聚合物多孔膜中对许多样品进行了微蛋白电泳,因此可以自动同时进行分离分析;解决方案:启动该设备时,支撑液会自动被缓冲液润湿,将固定量的样本施加到被缓冲液润湿的载体上,施加到载体上的间隔物迁移,由于迁移而出现在载体上的迁移图像被固定,载体中包含的蛋白质固定液洗涤后,对洗涤后的支持物上的迁移图像进行银染技术,将染色后的支持物脱色然后,将银粘附到脱色载体上的迁移图像上,并测量迁移图像的浓度。;版权所有:(C)2003,JPO

著录项

  • 公开/公告号JP2003215098A

    专利类型

  • 公开/公告日2003-07-30

    原文格式PDF

  • 申请/专利权人 JOKOH CO LTD;

    申请/专利号JP20020012240

  • 发明设计人 CHIBA TOMOHITO;

    申请日2002-01-22

  • 分类号G01N27/447;C12M1/00;C12M1/34;G01N33/483;G01N33/68;

  • 国家 JP

  • 入库时间 2022-08-22 00:16:16

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