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OLIGONUCLEOTIDE PRIMER FOR SPECIFICALLY DETECTING SULFATE-REDUCING BACTERIUM IN BIOCONSORTIA SAMPLE

机译:寡核苷酸底漆,用于特异性地检测生物降解菌样品中的硫酸盐还原菌

摘要

PROBLEM TO BE SOLVED: To provide oligonucleotide primers for PCR capable of specifically detecting a bacterium of the genus Desulfovibrio and a sulfate-reducing bacterium related to the bacterium and to provide a method for specifically detecting the bacterium of the genus Desulfovibrio in various environmental samples and artificial environmental samples such as reserved crude oil and the bacterium related to the bacterium by using the primers.;SOLUTION: A pair of oligonucleotide primers which are specific to 16S rRNA sequence of the bacterium is prepared. A bacterium of the genus Desulfovibrio in various environmental samples and artificial environmental samples such as stored crude oil and the sulfate-reducing bacterium which is related to the bacterium is specifically detected in a PCR method by using the primers.;COPYRIGHT: (C)2004,JPO
机译:解决的问题:提供用于PCR的寡核苷酸引物,其能够特异性地检测脱硫弧菌属细菌和与该细菌有关的硫酸盐还原细菌,并提供一种在各种环境样品中特异性检测脱硫弧菌属细菌的方法。人工环境样品,例如保留的原油和与细菌有关的细菌,使用引物。解决方案:制备一对对细菌的16S rRNA序列特异的寡核苷酸引物。使用引物通过PCR方法专门检测各种环境样品和人工环境样品(如储存的原油和与该细菌有关的硫酸盐还原细菌)中的Desulfovibrio属细菌.COPYRIGHT:(C)2004 ,日本特许厅

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