Method for detecting the reactivity of lymphocyte in blood to specific antigen

摘要

The present invention discloses a method for detecting the reactivity of lymphocyte in blood to a specific antigen, wherein the standard cell strains expressing a known HLA antigen respectively is cultured with the recipient's lymphocytes for 2-28 hours in vitro, and then the reactivity of the lymphocytes is determined through an immunological assay. The invention provides a method to monitor dynamically the recipient's immune status to donor organ after transplantation, and also provide a basis for adjusting the clinical immunosuppressant dosage administrated to the recipient, which can improve the success rate of organ transplantation significantly.