Method for cloning polyketide synthase genes

摘要

A method for obtaining “perfect probes” for type I modular polyketide synthase (PKS) or non-ribosomal peptide synthase (NRPS) gene clusters enables the identification of all such gene clusters in a genome. By sequencing small fragments of a random genomic DNA library containing one or more modular PKS or NRPS gene clusters, and identifying which fragments emanate from PKS or NRPS genes and knowing the approximate sizes of the genome and the target gene cluster, one can predict the frequency that a PKS or NRPS gene fragment will be present in the library sequenced.