Detection of hybridization on oligonucleotide microarray through covalently labeling microarray probe

摘要

A method for detecting hybridization between a probe with a free 3′ end on an oligonucleotide microarray and a polynucleotide from a sample is disclosed. The method involves contacting the sample with the probe under a hybridization condition under which desired hybridization events occur, performing an elongation reaction on the probe using at least one labeled nucleotide, removing unincorporated labeled nucleotides from the microarray, and determining whether the probe is labeled.