Highly immunogenic protein against the intracelluar pathogen agent Piscirickettsia salmonis, which affects salmon culture, amino acid and nucleic acid sequences of said protein and its application in the development of useful methods for the prevention and diagnosis of diseases caused by said pathogen

摘要

The present invention relates to processes for purifying a highly immunogenic protein derived from naturally infected fish tissues with the intracellular bacterium agent Piscirickettsia salmonis; said novel protein is named CHAP, and is further characterized by its amino acid and nucleic acid sequence which are in turn useful for obtaining the protein full length through genetic engineering.; Bidirectional DNA sequencing of chap revealed that the gene contained a single open reading frame encoding 545 amino acid residues with a predicted molecular mass of 58.5 and 4.9 isoelectric point. ;This inmunodominant antigen is intended for use in vaccines against Piscirickettsia salmonis who affects salmonidae species such as coho and atlantic salmons and rainbow trout.