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Engineering of living cells for the expression of holo-phycobiliprotein-based constructs
Engineering of living cells for the expression of holo-phycobiliprotein-based constructs
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机译:活细胞工程化,以表达基于全藻胆蛋白的构建体
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摘要
Recombinant cells which express a fluorescent holo-phycobiliprotein fusion protein and methods of use are described. The cells comprises a bilin, a recombinant bilin reductase, an apo-phycobiliprotein fusion protein precursor of the fusion protein comprising a corresponding apo-phycobiliprotein domain, and a recombinant phycobiliprotein domain-bilin lyase, which components react to form the holo-phycobiliprotein fusion protein. Also described are holo-phycobiliprotein based transcription reporter cells and assays, which cells conditionally express a heterologous-to-the-cell, fluorescent, first holo-phycobiliprotein domain.
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