Proteases having modified amino acid sequences conjugated to addition moieties

摘要

Protease conjugates such as subtilisin conjugates are provided comprising a protease moiety and one or more addition moieties wherein the protease moiety has a modified amino acid sequence of a parent amino acid sequence. The parent amino acid sequence comprises a first epitope region, a second epitope region and a third epitope region, and the modified amino acid sequence comprises a substitution by a substituting amino acid at one or more positions in one or more of the epitope regions. When a substitution occurs in the first epitope region, the substitution occurs at one or more positions corresponding to positions 70-84 of subtilisin BPN. When a substitution occurs in the second epitope region, the substitution occurs at one or more positions corresponding to positions 103-126 of subtilisin BPN. When a substitution occurs in the third epitope region, the substitution occurs at one or more positions corresponding to positions 217-252 of subtilisin BPN. Each addition moiety is covalently attached to one of the substituting amino acids. Cleaning and personal care compositions are provided containing the protease conjugates which have reduced immunogenicity relative to their corresponding parent proteases.