Lysophospholipase, method for its production, a Nucleic Acid Sequence, a recombinant expression vector, a recombinant host, Process for acilos groups Hydrolyze Fatty and process to improve the ability of Filtering a suspension or solution

摘要

Lisofosfolipasas have been isolated from Aspergillus niger (. And.Oryzae) with molecular masses of approximately 68 kDa and amino acid sequences of 600 604 amino acid residues and a method for its production, which include: (a) a polypeptide encoded by Coding lysophospholipase a part of DNA Sequence cloned in a plu00e1smid This deposit or in Escherichia coli Number DSM 13003, DSM DSM DSM 13083 13082 13004, or,Or (b) a polypeptide which has an amino acid sequence of the mature Peptide is shown in SEC ID no: 2, 4, 6, or 8, or may derive from the same substitution, deletion and / or insertion of one or more amino acidsIn particular, by deletion of 25 - 35 amino acids in the C Terminus; (c) a similar polypeptide defined in (a) or (b): (i) is at least 70% Homology with said polypeptide, (ii) is immunologically reactive with an Antibody against the polypeptide produced in Purify A,Or (iii) is an allelic variant of this polypeptide; or (d) a polypeptide is encoded by a Nucleic Acid Sequence that hidrida under conditions of high Severity with a complementary Strand of Nucleic Acid Sequence which shows how the nucleotides of sec id 109 - 1920 No: 1; 115 - 1914 SEC ID no: 3; 70 - 1881 SEC ID no: 5; or 193 - 2001 SEC ID no: 7The same or a Subsequence that has at least 100 nucleotides. The New lisofosfolipasas have only limited Homology to known amino acid sequences. In addition to the new isolated genes encoding enzymes and cloned into E. coli strains.Further described a recombinant expression vector, a recombinant host Cell, a process to Hydrolyze Fatty acyl groups in a phospholipid and a process to improve the ability of Filtering a suspension or aqueous origin carbohydrate cont Contains phospholipid.