Quality indicator of earthworm humus and organic resu00ecduos compounds by electron paramagnetic resonance spectroscopy

摘要

"Quality indicator of earthworm humus and organic resu00ecduos compounds by electron paramagnetic resonance spectroscopy".The production of humus in Brazil has experienced a significant growth in recent years.And that an appreciable amount of producers of humus has appeared on the market with a business opportunity for the rural producer.However, the lack of standards and methods for qualification of this material have brought losses to both farmers and consumers.The degree of humificau00e7u00e3o soil organic matter is a feature used to estimate the extent of transformation of organic matter in the organic soils and minerals.A tentative method used to determine the degree of humificau00e7u00e3o of humic substances, the main constituents of soil organic matter.It is the determination of the level or concentration of free radicals like semiquinona, measured through the technique of electron paramagnetic resonance (EPR).Based on this fact, whereas the concentration of free radicals semiquinona, detectable by EPR technique is associated with the process of humificau00e7u00e3o,And the humus produced correctly has a level well characterized of these radicals.Is that claim the following application of intellectual protection: determining the quality of the humus and organic compounds can be obtained by the resonance spectroscopy paramagnu00e9tica ElectronicsThrough the determination of the following parameters: concentration of free radicals like semiquinona, row width of the signal.The measure of factor G and the characteristic of the base line in spectra obtained.For proper observation of the parameters cited the following conditions in the experiment of EPR should be guaranteed: microwave power less than or equal to 2 MW; modulation amplitude equal to or mainR 2 Gauss peak to peak; base line proper, i.e. closer to linearity and the ambient temperature.These parameters are intended to avoid distortions in the signals which may lead to errors in the determination of the concentration (in case the power of microwave and the quality factor Q of the cavity snoresNTE), and changes in the width of the line of the signal (due to the modulation amplitude high, as normally used is 1 / 3 or less the width of the line's intrinsic signal of interest).Generally, 30 mg of the sample are sufficient to carry out the measures.For quantification of the concentration of free radicals semiquinona will be used the method secondary standard with the ruby crystal (Al ~ 2 ~ ~ 3 ~) containing 0.5% Cr ^ + 3 ^ change weight.Your factor G is 1263 and, therefore, does not interfere with the signal of the free radical semiquinona, whose value of G is around 2003.