The transformation process and co genetic transformation of yeasts.

摘要

"The process of transformation and co genetic transformation of yeast.The present invention is vectors that can be used to transform a yeast strains yeast prototru00f3ficas laboratory isolated from industrial processes.And biodiversity, the environment, belonging to the genus Saccharomyces or other genera of yeasts are called "non Saccharomyces, formed by rDNA fragments EcoRI - EcoRI 2.1KB of flash of S. cerevisiae that include the gene called 5S and the spacers NTS1 and nuts2.They were thus constructed vector F1 - 9g18, with the cassette of gene expression of glicoamilase of Aspergillus awamory cloned in place hpal present in the spacer NTS1, the vector fa - 4.With the gene that confers resistance to geneticina (G418) cloned in hpal site present in the spacer NTS1,And the fgg7 vector with the gene that confers resistance to geneticina (G418) cloned in hpal site present in the spacer NTS1 and the cassette of gene expression of glicoamilase of Aspergillus awmory cloned nThe site hindlll present in the spacer NTS1.