Cryogenic preservation of biologically active material using high temperature freezing

摘要

"Cryogenic preservation of biologically active material using freezing of high temperature.The present invention relates to a viable biological material that is cryogenically preserved (cryopreservation) to chemically preparing material for freezing.Immersing the material in a fluid tank for cooling.And circulate the cooling fluid below the material to a predetermined substantially constant speed and temperature to freeze the material.A method according to the present invention freezes the biological material fast enough to prevent the formation of ice crystals in the interior of the cellular structures (vitrification).The temperature of the cooling fluid is the preferred form between - 20 and - C 198 198 30 C,It is warm enough to minimize the formation of stress fractures in the cellular membranes due to thermal changes.The frozen cells by using the method according to the present invention proved to be endowed with a coefficient of survival of 80 per cent.Which is significantly higher than the other methods of cryopreservation.