I stor grad immunogent protein mot det intracellul¶re patogene middelet Piscirickettsia salmonis, som påvirker oppdrettslaks, enaminosyresekvens og nukleinsyrer av nevnte protein, og dets anvendelse ved utvikling avnyttige fremgangsmåter til å

摘要

The present invention relates to processes for purifying a highly immunogenic protein derived from naturally infected fish tissues with the intracellular bacterium agent Piscirickettsia salmonis; said novel protein is named CHAP, and is further characterized by its amino acid and nucleic acid sequence which are in turn useful for obtaining the protein full length through genetic engineering.; Bidirectional DNA sequencing of chap revealed that the gene contained a single open reading frame encoding 545 amino acid residues with a predicted molecular mass of 58.5 and 4.9 isoelectric point. This inmunodominant antigen is intended for use in vaccines against Piscirickettsia salmonis who affects salmonidae species such as coho and atlantic salmons and rainbow trout.