Method to detect male antifertility problems

摘要

Method to detect male anti-fertility problems based on the determination of latent phospholipid hydroperoxide glutathione peroxidase (PHGPx) comprising the steps of: (a) Obtaining a sperm sample; (b) Solubilising the spermatozoa by using detergents and chaotropic agents and reactivating latent PHGPx by using high concentrations of thiols, wherein the thiol concentration is sufficient to fully dissolve the sperm mitochondrial capsule; and (c) Determining enzymatic activity of reactivated latent PHGPx.