Methods for Synthesizing conjugated polypeptide insulin and proinsulin polypeptide oligomer Conjugates, and Methods of synthesizing the - conjugated oligomer

摘要

It relates to a method for Synthesizing a conjugated polypeptide insulin oligomer comprising: a) contacting a polypeptide comprising a polypeptide of proinsulin to insulin or joinedNo More peptides or peptides can be separated by links to produce insulin oligomer with a polypeptide that includes a Hydrophilic Group and lipophilic Group under condition sEnough to unite the oligomer to the portion of the polypeptide polypeptide insulin proinsulin and insulin - producing a polypeptide conjugate oligomer and (b) Separating one or more peptides of COnjugado to produce conjugated polypeptide insulin oligomer.The Step a) is carried out under conditions sufficient to produce activated oligomer capable of joining a nucleophilic functionality in proinsulin polypeptide.The polypeptide insulin has a polypeptide chain to chain B and One Where the peptides include a Connector and a First end of the c-terminal polypeptide chain is attached to a and BThe Second end of the n-terminal polipetido Chain.Also described is the Connector C - peptide and polypeptide that lacks lysine residues and have a First end terminal amino acid residue in the Conjugates and Methods for Synthesizing polyprEptido C - peptide oligomer