BIOACTIVE CONCENTRATE, THEIR PRODUCTION PROCESS AND CERTAIN MEDICINES THAT ALSO CONTAIN CHONDROITIN SULFATE

摘要

PCT No. PCT/RO96/00003 Sec. 371 Date Oct. 3, 1997 Sec. 102(e) Date Oct. 3, 1997 PCT Filed Mar. 14, 1996 PCT Pub. No. WO96/32117 PCT Pub. Date Oct. 19, 1996This bioactive concentrate is an active substance-aqueous solution or lyophilized powder-consisting of anti-hyaluronidase and antiinflammatory mucopolysaccharide polymers and an addition of restitutive and anti-hyaluronidase chondroitinsulphate, which has a pH=4-6 and antihyaluronidase activity. The bioactive concentrate producing method consists in a two-phased treatment of animal-originated connective cartilaginous tissues such as bovine and sheep trachea, umbilical cord, young animal tendons, bowels, testicles or sea organisms, with phenol solution and the solution resulted is concentrated by vacuum evaporation and then defatted; after filtering, the supernatant obtained is treated for deproteinization, the ion excess is removed by passing through ion exchanger column, the alcohol solution is concentrated to remove alcohol until reaching a volume of 70% as against the aqueous solution subject to proteinzation. Drug compsitions contain the bioactive concentrate-solution or in lyophilized state-associated with synergic substances such as: heparin, sodium or lysin acetylsallicylate, ascorbic acid, vitamin E,"I"-like structured water, benzyl alcohol, propylene glycocou, plant extracts (Achillea, Calendula, Matricaria, Plantago, Hypericum) and usual excipients, being conditioned in the form of intramuscular or intraarticular injections, ointment, gel and suppositories. Finally the histamines are removed and chondroitin sulphate is added up to the concentration required.