Method for increasing the accumulation of sterols in s. cerevisiae by transforming a mutant.

摘要

SE describes a method to increase the accumulation of squalene and sterols SPECIFIC in yeast, comprising INCREASING THE LEVEL OF EXPRESSION OF THE CODE OF A GENE STRUCTURAL a polypeptide having the activity of HMG-CoA to a mutant yeast having EFFECTS single or doubles in the expression of BISINTETICENZIMAS ESTEROL. LEVEL OF EXPRESSION OF GENE STRUCTURAL INCREASED PREFERABLY by transforming the yeast with a recombinant DNA molecule comprising a vector operably linked to a segment exogenous DNA encoding a polypeptide having the activity of HMG-COA and a promoter It is useful to drive expression of modified peptide transformed yeast. Polypeptide activity of HMG-CoA reductase enzyme is preferably an HMG-COA ACTIVE truncate. ALSO DESCRIBED recombinants DNA MOLECULES USEFUL TO TRANSFORM YEAST AND YEAST MUTANT TRANSFORMADASCON BLISS recombining DNA molecules.