REDUCTION OF NON-SPECIFIC HYBRIDIZATION THROUGH NEW BASE PAIRING COMBINATIONS.

摘要

METHODS ARE PROVIDED FOR SUBSTANTIALLY REDUCING FUND SIGNS FOUND IN NUCLEIC ACID HYBRIDIZATION TESTS. THE METHOD IS ESTABLISHED AS A PREMISE IN THE ELIMINATION OR SIGNIFICANT REDUCTION OF THE NON-SPECIFIC HYBRIDIZATION PHENOMENON, TO PROVIDE A DETECTABLE SIGNAL THAT IS PRODUCED ONLY IN THE PRESENCE OF THE TARGET POLYUCLEOTIDE OF INTEREST. IN ADDITION, A NEW METHOD IS PROVIDED FOR THE CHEMICAL SYNTHESIS OF ISOGUANOSINE OR 2 '' - DESOXI-ISOGUANOSINA. THE INVENTION ALSO HAS APPLICATIONS IN ANTISENTIDE AND APTAMERA THERAPEUTICS AND PHARMACOS MEETING.