METHOD OF TYPE-SPECIFIC DETECTION OF HERPES SIMPLEX VIRUS

摘要

The present invention relates to a method of the type-specific detection of herpes simplex virus, characterized bycomprising the step of DNA amplification in a liquid mixturecontaining either of a combination of a first DNA primercontaining an oligonucleotide having at least 15 bases in a basesequence of the formula (1a)CACGGGTATA AGGACATCCA (1a)with a second DNA primer containing an oligonucleotide having atleast 15 bases in a base sequence of the formula (2a):GGGTCCTCGT CCAGATCGCT (2a),or a combination of a first DNA primer containing anoligonucleotide having at least 15 bases in a base sequence ofthe formula (1b):GCCTCTTTTC CCCCGGGGAG (1b)with a second DNA primer containing an oligonucleotide having atleast 15 bases in a base sequence of the formula (2b):GGGAAAAAAG CCGCGCCGGGG (2b),a DNA polymerase and an aqueous liquid sample; and then the stepof DNA examination on the resultant reaction solution.According to the present invention, it is possible todifferentiate infectious disease caused by the HSV I or HSV II,accurately and rapidly in a type specific manner.