A METHOD OF PCR BASED DETECTION AND IDENTIFICATION OF RICE BLAST FUNGUS MAGNAPORTHE GRISEA

摘要

A method for detection and identification of infestation of tissue of rice with a virulent strain of Magnaporthe grisea. The method involves isolating deoxyribonucleic acid (DNA) from infected tissue, subjecting the isolated nucleic acid to amplification by polymerase chain reaction (PCR) using sets of primers complementary in base sequence to mif23 (GeneBank accession number GI 4732021), an infection specific gene of M. grisea specific to virulent strains of the fungus, to form a product containing amplified M. grisea DNA, detecting the presence of the amplified DNA separated from M. grisea DNA after electrophoresis. The invention also relates to a method of deriving DNA of M. grisea from plant tissue for amplification by PCR. The invention further relates to a kit for detection and identification of infestation of tissue of rice with a virulent strain of Magnaporthe grisea.