METHODS FOR DEPLETING AND ISOLATING ALLOREACTIVE AND ANTIGEN-REACTIVE T CELLS FROM HEMATOPOIETIC DONOR CELLS

摘要

The invention provides methods for depleting alloreactive or antigen-reactive T cells from a hematopoietic donor cell population, by co-culturing a recipient-derived dendritic cell population or cultured macrophage population with the donor cell population, under conditions wherein the recipient-derived population activates donor T cells in the donor cell population, and killing or removing the activated T cells. Also provided are hematopoietic donor cell population depleted of, or enriched for, alloreactive or antigen-reactive T cells prepared by these methods. The invention further provides methods of using the depleted and isolated hematopoietic donor cell population in transplant procedures.