METHODS FOR GENERATING DOUBLED HAPLOID MAIZE PLANTS

摘要

In one aspect, the present invention provides methods for generating doubled haploid and/or haploid maize plants from microspores. The methods of this aspect of the invention include the steps of: (a) selecting maize plant material comprising maize microspores at a developmental stage amenable to androgenic induction; (b) incubating the microspores in incubation medium at a temperature and osmolarity effective to induce androgenesis to obtain temperature-treated microspores; (c) isolating the temperature-treated microspores; (d) cultivating the isolated, temperature-treated microspores in cultivation medium with either at least one live plant ovary and/or ovary-conditioned medium to produce regenerative maize tissue, wherein the cultivation medium has an osmolarity between about 300 mOsm and about 500 mOsm and comprises at least one cytokinin and at least one auxin; and (e) regenerating maize plants from the regenerative maize tissue. The present invention also provides methods for producing regenerative maize tissue from maize microspores.