DEFINED SEQUENCE SINGLE STRAND OLIGONUCLEOTIDES INCORPORATING REPORTER GROUPS, PROCESS FOR THE CHEMICAL SYNTHESIS THEREOF, AND NUCLEOSIDES USEFUL IN SUCH SYNTHESIS

摘要

single strand oligonucleotides and defined sequence having a length of less than 200 units units include nucleotide pyrimidine or purine base modified and can groreporter mentioned above easily detectable attached to substituents on the modified nucleotide units.each panel is attached to the base of the respective nucleotide unit on a site of this sterically tolerantfor example, the c-5 position of the pyrimidine nucleotide and the c-8 position of purine nucleotide.such oligonucleotides are useful in the tracing.the isolation and detection of nucleic acid sequences of interest in additional cellular or acellular.a process for the chemical synthesis of oligonucleotides to single strand having a predetermined number of units based on pyrimidine nucleotide changes and / or in a su00e9que purine- on the basis of a predetermined, easily detectable reporter groups are established on sites sterically tolerant through substituentseither before or after the incorporation of such units in the chain of components is also described.novel nucleosides useful in the chemical synthesis of oligonucleotides with single strand and defined sequence are also described.