A HOMOGENOUS METHOD FOR DETECTION OF A POLYNUCLEOTIDE, WITH E.G. CLEAVABLE LANTHANIDE CHELATE LABEL

摘要

The present invention relates to a homogenous method for detecting at least one target polynucleotide in a sample comprising the steps of contacting a sample comprising said polynucleotide or polynucleotide forming nucleic acids or derivatives thereof, with labelled oligonucleotide probe under conditions where said oligonucleotide probe selectively hybridizes to said target polynucleotide, wherein the signal of the label increases or decreases upon the hybridization between the oligonucleotide probe and the target polynucleotide, exciting the label, and monitoring the signal intensity of said label to indicate the presence or absence in a sample of said target polynucleotide. According to the invention, said label is measured by time resolved fluorometry.