DETECTION OF SPECIFIC DINUCLEOTIDES IN DNA-SAMPLES BY FLUORESCENCE RESONANCE ENERGY TRANSFER (FRET)

摘要

The invention relates to a method for detecting specific dinucleotides in a DNA-sample. A polymerase-chain reaction (PCR) is carried out by using a) a nucleotide, which is part of the dinucleotide which is to be detected, wherein an adequate quantity thereof is marked by a donor-fluorphore and b) another nucleotide, which is part of the dinucleotide which is to be detected, wherein an adequate quantity thereof is marked with an acceptor-fluorophore. Said method determines or quantifies the presence of the dinucleotide by measuring the dimensions of the fluorescence resonance energy transfer (FRET) between the donor- and acceptor-fluorophore.