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E-GENECHIP ONLINE WEB SERVICE FOR DATA MINING BIOINFORMATICS

机译:用于数据挖掘生物信息学的E-GENECHIP在线Web服务

摘要

A method for data analysis of microarrays. The method includes accessing a software program that performs a multistage analysis of the biological image, through an internet webserver. The analysis includes at least a step of comparing the digitized quantitative data for each sample. The method may further include digitizing the data for each sample and quantitating the data for each sample. Further, the method may include using a software program which quantitates the intensity and size of each sample; compares the quantitative value for each sample with the quantitative value of one or more controls; captures data, quantitates data, analyses data, or stores data; removes the background based on negative controls; averages or adjusts intensities as a function of the number of the biological images; generates reports; and stores data. The microarray can be processed to display the samples using an assay such as chromogenic assay or fluorescent assay, using a radioactive label imaged on radiographic film or using any other means in the art. The samples can be oligonucleotides such as DNA or mRNA or proteomics. The biological images can be in any form, preferably in the form of immunoassays, dot blots, Northern assays, Southern assay, Western assay, and electrophoretic gels.
机译:一种微阵列数据分析的方法。该方法包括通过互联网网络服务器访问执行生物图像的多阶段分析的软件程序。该分析至少包括比较每个样品的数字化定量数据的步骤。该方法可以进一步包括数字化每个样本的数据和量化每个样本的数据。此外,该方法可以包括使用软件程序,该软件程序量化每个样品的强度和大小;以及将每个样品的定量值与一个或多个对照的定量值进行比较;捕获数据,量化数据,分析数据或存储数据;根据负面控制移除背景;根据生物图像的数量对强度进行平均或调整;生成报告;并存储数据。可以使用诸如生色测定或荧光测定之类的测定法,使用在射线照相胶片上成像的放射性标记或使用本领域中的任何其他手段来处理微阵列以显示样品。样品可以是寡核苷酸,例如DNA或mRNA或蛋白质组学。生物学图像可以是任何形式,优选地是免疫测定,斑点印迹,Northern测定,Southern测定,Western测定和电泳凝胶的形式。

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