the invention relates to a process of directed mutagenesis of dna. in this method, a parental dna containing a unique restriction site, is used to generate the dna containing the desired mutation progeny with no restriction site.parental dna and non dna mutant progeny are treated with an enzyme mutant form, provided that it is at the level of dna restriction site to divide the parental non divided, leaving the mutant dna.the dna of the mutant was selected by progeny cells by dna treated with the enzyme under conditions where the cells are transformed with the dna is not divided into higher yields than with dna is divided in such a way that the majority of transformants are the mutant dna.
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