METHOD FOR DETECTING MUTATIONS, INSERTIONS, DELETIONS AND POLYMORPHISMS ON DNA AND THE USE THEREOF

摘要

The inventive method for detecting mutations, insertions, deletions and polymorphisms on DNA is characterized in that a first specific PCR reaction is carried out with a primer pair which flanks the position of the genome which is to be examined, wherein a respectively different universal oligonucleotide sequence, which is not complementary with the DNA sequence to be examined, is also joined to the 5'-end of each primer; a second universal PCR reaction is carried out with a marked primer pair which is complementary to the universal oligonucleotide sequences used in the first PCR.