USE OF ACTIVE SUBSTANCES FOR THE PROPHYLAXIS AND/OR TREATMENT OF DISEASES CAUSED BY CELL GROWTH DISTURBANCES AND TEST SYSTEM FOR DISCOVERY OF SAID ACTIVE SUBSTANCES

摘要

The invention relates to a method for screening of substances which modulate the interaction of Nlk in the COP9 signalsome complex, whereby a) a test system comprising a further member of the COP9 signalsome complexes different from Nlk or all further members of the COP9 signalsome complex and, optionally, one or several molecules naturally dependent on at least one of the further members of the COP9 signalsome complex, is incubated with a prospective modulating substance or a mixture of such substances and also incubated with Nlk, if Nlk is not present in the test system, b) after an optional washing stage to remove further members of the COP9 signalsome complex not bonded to Nlk, the activity or concentration of at least one member of the COP9 signalsome complex, or a molecule naturally dependent thereon is determined, however not Nlk, c) a substance or a mixture of substances are selected when the activity or concentration measured in step b) is elevated or depressed relative to the execution of steps a) and b), however, without Nlk or with mutated Nlk.