PHYSIOLOGICAL MEDIUM FOR PERFUSING, PRESERVING AND STORING ISOLATED CELL, TISSUE AND ORGAN SAMPLES

摘要

A physiological liquid medium is provided having the basic, synergistic components to allow its universal application in preserving cellular and functional integrity in vitro of different cell, tissue and organ types isolated from different mammalian species. The medium comprises an aqueous solution in sterile purified water of: i) a salt component comprising: a) from 100 to 150 mmoles/L of sodium ions, b) from 2.5 to 6.2 mmoles/L of potassium ions, c) from 1.0 to 2.5 mmoles/L of calcium ions, d) from 0.4 to 25 mmoles/L of magnesium ions, and e) from 96 to 126 mmoles/L of chloride ions; ii) a buffer component comprising: f) from 21 to 27 mmoles/L of bicarbonate ions, and g) from 1 to 12 mmoles/L of TES, MOPS or BES; iii) a substrate component comprising: h) 2 to 11 mmoles/L of glucose, I) 50 to 150 νmoles/L of glycerol and j)7 zo 15 νmoles/L of choline; iv) an amino acid component comprising: k) 5 to 400 νmoles/L of glutamate, l) 5 to 200 νmoles/L of aspartate and m) 100 to 2000 νmoles/L of glutamine; v) a co-enzyme component comprising: n) 1 to 120 nmoles/L of thiamine cocarboxylase; vi) a vitaminoid component comprising: o) 40 to 70 νmoles/l of D- or DL- or L-carnitine; vii) a protein component comprising: p) 5 to 200 m I.U./L of porcine or human insulin.