TRANSPOSON FOR BIDIRECTIONAL INTRAMOLECULAR GENOME DELETIONS, CONSTRUCTION OF NOVEL MICROORGANISM AND IDENTIFICATION OF NONESSENTIAL GENES USING THE SAME

摘要

Disclosed is a deletion method of an optional region in genome using an intramolecular transposition of transposon, and selection markers. Particularly, disclosed are a transposon having transposase recognition sites (outer element, OE) and selection markers; and methods for identifying nonessential genes for growth under the given conditions and constructing a novel strain, by inserting the transposon into a genome of a microorganism and deleting an optional site bidirectionally using a transposase expression vector.