Increase of the expression levels of factor VIII by insertion of spliceable nucleotide sequences into factor VIII cDNA

摘要

A modified factor VIII cDNA is disclosed, wherein in introns 1 and/or 13 of the wild-type factor VIII cDNA one or more splicable nucleotide sequences or a nucleotide sequence which will be spliced during the export of the pre-mRNA from the nucleus have been inserted. Further a process for the production of a biologically active recombinant human factor VIII or its derivative is disclosed, which is performed by cultivating an animal cell line comprising a recombinant expression vector containing said modified factor VIII cDNA. Moreover a transfer vector for use in the human gene therapy is described which comprises said modified factor VIII cDNA.