Use of mutated recognition sites for multiple successive recombinase-mediated recombinations in a genetic system

摘要

The present invention relates to an improved recombinant techniques for the site-specific recombinase-mediated recombination using the mutated recognition sequences. In this case, making use of a non-identical pair of recognition sequence mutants. Each of the recognition sequence mutants is composed of two recognition sequences, which are separated by a spacer. At a recognition sequence mutations were introduced so that after recombination by a sequence-specific recombinase a recognition sequence mutant is formed which is no longer recognized by the recombinase.