H- OVEREXPRESSION METHOD OF RECOMBINANT HUMAN H-CHAIN FERRITIN IN PICHIA PASTORIS AND THERAPEUTIC COMPOSITION FOR ANEMIA USING THE SAME

摘要

PURPOSE: Provided are an overexpression method of recombinant human H-chain ferritin(rHN) in Pichia pastoris and a therapeutic composition for anemia using the same which is excellent in storing iron. CONSTITUTION: Recombinant Pichia pastoris(KCCM 10279) is obtained by transformation using an expression vector pGAPZ alpha A/rHN derived from Pichia pastoris and containing human H-chain ferritin(rHN) genes which are amplified by using a pair of primers represented by the SEQ ID NOs:2 and 3. The human recombinant rHN is a homopolymer consisting of 24 H-chains having 24000 Da, and its molecular weight is 576000 Da.