Recombinant adenoviral vector expressing HPV-like particles and live vaccine for cervical cancer prepared therefrom

摘要

PURPOSE: Provided are a recombinant Adenovirus vector producing human Papilomavirus-like particles(HPV VLP) and vaccine containing the HPV VLP as an active ingredient for the prevention and treatment of cervix cancer. CONSTITUTION: The recombinant vector, RAdBL2, includes a human Papilomavirus(HPV) gene and the Adenovirus genome deleted from an E1 gene, and produces human Papilomavirus-like particles(HPV VLP) in both growth-permissive cells ad growth-non-permissive cells, wherein the HPV gene is characteristically one of HPV-16 L1, HPV-16 L2, HPV-18 L1 or HPV-18 L. The recombinant vector, RAdBL2, is manufactured by cotransfection of a plasmid including an HPV gene and the Adenovirus genome deleted from an E1 gene, then by in vivo recombination. The vaccine for the prevention and treatment of cervix cancer contains, as an active ingredient, the HPV VLP produced by the recombinant vector, RAdBL2.